NucleoBond® Xtra Midi / Maxi Transfection-grade plasmid DNA 60% time-saving, 100% increased yields Features The new generation of ultra-fast and high-yield, transfection-grade plasmid DNA purification at Midiprep and Maxiprep scale NucleoBond Xtra Midi Column • Optimized column design: plasmid Midiprep in approximately 30 min, plasmid Maxiprep in 35 min • Column filter included: high filter flow rates, parallel lysate clearing and loading onto the column • Improved silica material: typical yields of 250 μg (Midi) and 1 000 μg (Maxi) • Transfection-grade plasmid DNA due to patented anion-exchange technology • NucleoBond® Xtra Plus kits contain NucleoBond® Finalizers to speed up DNA precipitation NucleoBond® Xtra Midi NucleoBond® Xtra Maxi Technology Anion-exchange chromatography Anion-exchange chromatography Format Midi gravity flow columns Maxi gravity flow columns Lysate clarification Column filters Column filters Sample material < 200 mL (high copy) < 400 mL (low copy) < 600 mL (high copy) < 1200 mL (low copy) Vector size < 300 kbp < 300 kbp Typical yield 250 μg 1000 μg A260/A280 1.80–1.95 1.80–1.95 Isopropanol precipitation Xtra Midi Centrifugation Xtra Midi Plus NucleoBond® Finalizer Xtra Maxi Centrifugation Xtra Maxi Plus NucleoBond® Finalizer Large Preparation time 70 min/4 preps 30 min/4 preps 80 min/4 preps 35 min/4 preps Applications • High-copy plasmids from E. coli • Low-copy plasmids from E. coli* * Increased buffer volumes required. See "Ordering information"