Features PCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume • Two applications in one kit – one buffer with optimal performance for both applications • High recoveries for fragments down to 50 bp • Minimized elution volume of 15 μL – highly concentrated DNA • Binding buffer with pH indicator • Separate buffers for single stranded DNA or SDS containing samples available • Suitable for all gel buffer systems (e.g., TAE, TBE) • Also available for QIAcube® (please see "Ordering information" and contact Technical Service at techbio@mn-net.com for more information). Technology Silica-membrane technology Format Mini spin columns Sample material < 400 μL PCR reaction mixture < 400 mg TAE / TBE agarose gel Fragment size 50 bp–approx. 20 kbp Typical recovery 70–95% A260/A280 1.8–1.9 Elution volume 15–30 μL Preparation time 10 min/6 preps Binding capacity 25 μg Applications • Purification of PCR products • Extraction of DNA from agarose gels • DNA extraction from polyacrylamide gels • Clean-up of single stranded DNA and RNA extraction from agarose gels (Binding Buffer NTC required, not included in the kit, see “Ordering information”) • Clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the kit, see “Ordering information”) • Typical downstream applications: cloning, sequencing, PCR, restriction analysis • NucleoSpin® Gel and PCR Clean-up ensures complete removal of contaminations such as: - nucleotides, primers - enzymes - mineral oil - PCR additives (e.g., salts, betaine, DMSO) - detergents (e.g., Tween 20, Triton X-100) - dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti®-Safe GelStain, DNA SafeStain) Ordering information Product Preps Specification REF NucleoSpin® Gel and PCR Clean-up 10 NucleoSpin® Gel and PCR Clean-up Columns, Collection Tubes (2 mL), buffers 740609.10 50 as above 740609.50 250 as above 740609.250 240 Kit for use on QIAcube®* NucleoSpin® Gel and PCR Clean-up Columns, buffers 740609.240C Product accessories Pack of Specification REF Buffer NTB 150 mL for clean-up of SDS-containing samples 740595.150 Buffer NTC 100 mL for clean-up of single stranded DNA